Fig. 2

The niche breadth of the honey bee varied significantly over latitude, though the trends differed depending on whether we define niche breadth using the number of plant (A, C) or bacteria (B, D) ZOTUS identified in a honey sample. A Honey bees visited more plant ZOTUs towards the poles (i.e., broader niches), and C the strength of this trend was similar in the Northern (blue circles) and Southern (orange diamonds) hemispheres. B Honey bees encountered fewer bacterial ZOTUs towards the poles (i.e., narrower niches), though this trend was much weaker (but still significant) in the Southern hemisphere. The curves represent fitted values from a model including the log of the total number of reads to account for increasing detectability with increasing sequence yield. The solid line represents the fit for the mean number of reads for each taxonomic group and the dotted lines represent the 25% and 75% quantiles. For the mean number of reads, we also show results for each hemisphere separately