Fig. 5

The secretion function of Pi04314 signal peptide. Yeast YTK12 strains carrying the empty vector, the signal peptide of NH_1 and Avr1b grew on the CMD-W and YPRAA plates, respectively. The enzymatic activity of invertase was detected by the reduction of 2, 3, 5-triphenyl tetrazolium chloride (TTC) to insoluble red-colored 1, 3, 5-triphenyl formazan (TPF). The negative controls are the YTK12 strain and empty vector (EV), and the positive control is the YTK12 strain carrying the signal peptide of Avr1b