Fig. 4

Phylomorphospaces of (A) PC 1 and PC 2 of body shape; (B) PC 1 and PC 2 of head shape (trajectories of each PC are as described for Fig. 2), and (C) regression of body shape (Procrustes coordinates) on standard length versus standard length alone. The phylogeny recovered using concatenated BUSCO genes is projected into the morphospace (see Sidlauskas 2008). Taxonomic entities are color coded by macrohabitat group; blue = lacustrine; red = riverine; black = non-Sulawesi. Internal nodes of the phylogeny are colored white and provide estimates for ancestral states. Lacustrine and riverine habitat groups show clear disparity, despite close phylogenetic relationships. Red outlines indicate species tips and internal nodes of the clade restricted to the Malili lakes system, which was found be particularly diverse in body shapes. Riverine species are generally more similar to non-Sulawesi species, whereas lacustrine species readily colonized previously unoccupied morphospace regions. Backtransform morphospaces of the respective PC morphospaces are plotted as grey shapes into the background. These are based on convex hulls of landmark constellations, illustrate shape changes along the PC axes and represent body shape states at the plotting coordinates. Refer to Suppl. Fig. S3-5 for species labels of all data points