Table 2 List of analyses performed in the present study

18S-ITS-28S rRNA tree

136

130 Spirostomum + 1 Pseudoblepharisma + 5 Anigsteinia specimens

CO1 gene tree

50

49 Spirostomum + 1 Anigsteinia specimens

Concatenated tree

38

Concatenated data matrix of 265 protein-coding genes. 37 Spirostomum + 1 Anigsteinia specimens

Neighbor-net network

38

Concatenated data matrix of 265 protein-coding genes. 37 Spirostomum + 1 Anigsteinia specimens

ASTRAL species tree

38

Gene trees of 265 protein-coding genes were used to estimate the species tree under the MSC model.

37 Spirostomum + 1 Anigsteinia specimens

BPP 265 loci

38

Multiple sequence alignments (MSA) of 265 protein-coding genes were used to estimate the species tree

and species delimitation under the MSC model. 37 Spirostomum + 1 Anigsteinia specimens

BPP 100 loci

38

Multiple sequence alignments (MSA) of 100 protein-coding genes were used to estimate the species tree

and species delimitation under the MSC model. 37 Spirostomum + 1 Anigsteinia specimens