Fig. 3

Phylogenomic relationships of Spirostomum species from 38 specimens and 265 protein-coding genes. (A) Maximum likelihood (ML) tree topology of a phylogenomic matrix comprising 62,043 unambiguously aligned amino acid residues was inferred using IQ-TREE under the LG + C60 + F + G4 model. (B) ASTRAL species tree topology inferred from 265 gene trees. One Anigsteinia sp. was used as an outgroup. Numbers at nodes are ML bootstrap values and SH-aLRT from 1,000 replicates (IQ-TREE) and local posterior probability (LPP) for ASTRAL are given at the nodes. Red solid circle on nodes means maximum support. The scale bar corresponds to the number of nucleotide substitutions. (C = China; E = Europe; K = South Korea; U = USA)