Fig. 3

Genes with convergent substitutions of amino acids exhibiting similar biochemical characteristics in semi-aquatic mammals. The left side of the figure shows a phylogenetic tree of 42 species, with the ends of the branches representing their abbreviated Latin names, corresponding to the full names listed in Table 1. Yellow highlights denote branches of semi-aquatic mammals, while black vertical lines demarcate the semi-aquatic species into different evolutionary clades, indicating independently occurring secondary aquatic adaptations. The right side lists the amino acid positions with convergent substitutions: the 2152nd of CHD7, the 236th and 650th of GSDME, the 263rd of OTOP1, and the 404th and 744th of SLC26A2. An " × " with no background color indicates that the gene was not identified in this species, and this evolutionary branch was excluded from the analysis of that gene. The posterior probabilities for each convergent site are all greater than 0.95