Table 4 qPCR results from the experiment comparing eDNA detection using a traditional vacuum pump filtration system (VP) and the newly-developed WET system (WET). Ct: cycle threshold; TM: melting temperature
From: Citizen science in eDNA monitoring for Mediterranean monk seal conservation
System | Filter porosity (µm) | Mean Ct (SD) | Mean TM °C (SD) | % of positive replicates |
|---|---|---|---|---|
WET | 0.22 | 21.65 (0.16) | 71.78 (0.07) | 100% (10 out of 10) |
VP | 0.22 | 20.43 (0.59) | 71.89 (0.10) | 100% (10 out of 10) |
WET | 0.45 | 21.54 (0.10) | 71.84 (0.09) | 100% (3 out of 3) |
VP | 0.45 | 20.49 (0.16) | 71.83 (0.09) | 100% (3 out of 3) |