Fig. 6
From: Wing spot in a tropical and a temperate drosophilid: C = C enrichment and conserved thermal response

Raman spectral analysis of spotted (S) and unspotted regions (US) of wings of D. biarmipes and D. suzukii males. The analysis revealed distinct differences in the chemical composition between these two areas. The peaks indicate C = C richness in the spotted area of the wings; however, the same was not observed in the unspotted region of the same wing (data not shown) suggesting a chemical variation between the two different areas of the wings. The Raman spectroscopy results highlighted two different peaks for the spotted region (i.e., 1550 cm−1 and 1350 cm−1) indicating the presence of C = C and C = O respectively. The spectral analysis of the second region (i.e., the unspotted or the transparent region) did not show any clear peaks indicating an absence or lower concentration of the compound, further indicating a chemical heterogeneity between the spotted and the unspotted areas of the wings