Fig. 2

A. The tenascin-like predicted protein from the hemichordate Saccoglossus kowalevskii (XP_006814129.1). B. Multiple sequence alignment (in MUSCLE 3.8) shows alignment of the RGD-like motif lysine-glycine-aspartic acid (KGD) present in the fifth FN3 domain of the tenascin-like protein with the arginine-glycine-glutamic acid (RGE) motif from vase tunicate Ciona intestinalis tenascin and the RGD motifs of the Florida lancelet Branchiostoma floridae tenascin and chicken Gallus gallus TNC. Asterisks indicate identical residues. Conserved and semi-conserved residues are indicated with colons and periods, respectively. C. Multiple sequence alignment shows the first EGF-like domain of S. kowalevskii tenascin-like protein is a tenascin-type EGF-like domain. D. Conservation of the FReD of S. kowalevskii XP_002731388 with the FReD of H. sapiens TNR