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Table 4 Amplicon annotation results for the examined primers after pooling all samples

From: Development of environmental DNA metabarcoding primers for marine mollusks and comparison with published primers

Taxon

MollCOI253

Moll12S150

Moll12S195

Moll12S100

unionoida

veneroida

Aplacophora

0

0

0

0

0

0

Cephalopoda

0

1 (7, 0.00%)

0

0

0

0

Polyplacophora

1 (4,138, 3.54%)

0

0

0

0

0

Bivalvia

2 (611, 0.52%)

14 (400, 0.11%)

1 (9, 0.00%)

0

0

0

Gastropoda

15 (9,000, 7.71%)

1 (11, 0.00%)

0

0

0

0

Non-mollusk

70 (3,746, 3.21%)

1,036 (82,214, 23.39%)

101 (40,203, 7.08%)

6 (384,919, 100%)

324 (14,057, 10.05%)

406 (131,022, 58.17%)

Unassigned

397 (99,255, 85.02%)

6,979 (268,843, 76.49%)

850 (527,780, 92.92%)

0

23 (125,811, 89.95%)

1115 (94,199, 41.83%)

  1. Note: Numbers in table were expressed as number of MOTUs (Abundance of these MOTUs, proportion of all MOTUs)