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Table 3 High-throughput sequencing results based on environmental DNA amplification

From: Development of environmental DNA metabarcoding primers for marine mollusks and comparison with published primers

Primer name

Samples

Raw reads

Total (range)

MOTUs

Mollusks %

No. Annotated Mollusks

Source

MollCOI253

4

116,750 (23,747 − 35,831)

4,85

11.78%

12

Present study

Moll12S150

4

351,475 (83,344 − 92,908)

8,031

0.12%

3

Moll12S195

2

567,992 (198,732 − 369,260)

952

0.001%

1

Moll12S100

2

384,919 (159,907 − 225,012)

6

0

0

unionoida

2

139,868 (68,264 − 71,604)

347

0

0

veneroida

2

225,221 (109,582 − 115,639)

1,521

0

0

16SrRNA

6

461,185

-

26.69%

8

[15]

Ceph18S

19

9,936,758 (23,311 − 50,066)

-

-

15

[40]

Unionoida + veneroida

15

-

-

-

42

[16]

  1. Note: - meant information was not available in the publications