Table 1 Primers tested in this study
Target gene | Primer name | Primers (5’~3’) | Product size(bp) | Annealing Temperature | GC% | Source |
|---|---|---|---|---|---|---|
COI | MollCOI154 | F-TGGGGGTTTTGGTAATTGGT | 154 | 57ËšC | 45.00 | Present study |
R-TCAACCAGTACCAGCTCCA | 52.63 | |||||
MollCOI253 | F-GGAGTAGGAACTGGTTGGAC | 253 | 57ËšC | 55.00 | ||
R-CAGCTGCTAACACAGGCA | 55.56 | |||||
MollCOI255 | F-TGGAGCTGGTACTGGTTGA | 255 | 57ËšC | 52.63 | ||
R-GCCCCAGCTAAAACAGGTAT | 50.00 | |||||
12Â S | Moll12S100 | F-TTGTATACCGTCGTCGTCAG | 100 | 57ËšC | 50.00 | |
R-AGCTGCACCTTGATCTGAC | 52.63 | |||||
Moll12S150 | F-TATGCTTGCCGGGCAACT | 150 | 59ËšC | 55.56 | ||
R-ACGGCCATACACCAACTGA | 52.63 | |||||
Moll12S195 | F-GTATTGCCGTTGTCAGCTT | 195 | 56ËšC | 47.37 | ||
R-CCTTACTCCTAAGTTCACCTTC | 45.45 | |||||
16Â S | Moll16S | F-GTCCTGTGAATGGTTTGACGAG | 228 | 60ËšC | 50.00 | |
R-TTGCTGCCCCAGCCAAAAC | 57.89 | |||||
COI | COI204 | F-TGTTACAGCTCACGCATTTGTT R-CCGGTACCTGCACCTCTTTC | 204 | 60ËšC | 40.91 | [13] |
60.00 | ||||||
Sepi | F-CACCAGACATAGCCTTCC | 155 | 54ËšC | 55.56 | [14] | |
R-GCCAGCATGAGATAGATTAC | 45.00 | |||||
NADH | NADH | F-TCGAGCCATAGCTCAAACCA | 147 | 59ËšC | 50.00 | [12] |
R-GCGAGTGGTAGTGAAAGAGT | 50.00 | |||||
Cytb | NZMS | F-TGTTTCAAGTGTGCTGGTTT | 92 | 56ËšC | 40.00 | [11] |
R-CAAATGGGCTAGTTGATTCTTT | 36.36 | |||||
16Â S | 16Â S rRNA | F-TGAGCGTGCTAAGGTAGC | 360 | 60ËšC | 55.56 | [15] |
R-AGCCAACATCGAGGTCGC | 61.11 | |||||
unionoida | F-GCTGTTATCCCCGGGGTAR | 170 | 58ËšC | 61.11 | [16] | |
R-AAGACGAAAAGACCCCGC | 55.56 | |||||
veneroida | F-CSCTGTTATCCCYRCGGTA | 159 | 56ËšC | 52.63 | [16] | |
R-TTDTAAAAGCCGAGAAGACCC | 42.86 | |||||
18 S | Ceph18S | F-CGCGGCGCTACATATTAGAC | 173–235 | 61˚C | 55.00 | [17] |
R-GCACTTAACCGACCGTCGAC | 60.00 |