Fig. 3
From: The dilemma of underestimating freshwater biodiversity: morphological and molecular approaches
Distribution and community composition of freshwater nanofauna (A-B) and microfauna (C-D). A and C Dendrogram cluster showing the similarity (Jaccard index) of size class communities of the five sediment samples in regard to species richness based on incidence-based data (presence/absence) using UPGMA clustering. Number of freshwater ASV80-100% (grey bars) and number of morphotypes (pink bars) related to the major taxonomic protist groups are shown. Taxonomic groups correspond to class level in the PR2 database classification. B and D Heatmap of nano- and microfauna ASVs98-100% with a 98–100% sequence similarity and taxonomic clustering on predicted genus level. Shown are the first 35 most abundant genera (out of 43 nanofauna genera and 79 microfauna genera) with class and genus level. In several cases reference sequences were not assigned to genus level, thus, a higher taxonomic level is shown. Numbers within the heatmap correspond to the number of ASVs assigned to this genus per site. Taxonomic groups correspond to class and genus levels in the PR2 database classification. The sequential color code corresponds to the relative abundance of reads with a sequence similarity of 98–100% assigned to the respective genus to either the nano- or microfauna size class. Pink asterisks indicate, if genera could also be detected morphologically. Nanofauna protist drawings are adapted from literature [31, 68]. Microfauna protist silhouettes are from PhyloPic [69] contributed by Guillaume Dera, 2023 (CC0 1.0 Universal Public Domain Dedication) and Yan Wong, 2013 (Attribution 3.0 Unported, https://creativecommons.org/licenses/by/3.0/)