Fig. 4

Gel electrophoresis of PCR products demonstrating species-specific amplification of targeted species: a Saccostrea multiplex 1 PCR products of targeted Saccostrea species DNA amplified at an annealing temperature of 62 °C; b Saccostrea multiplex 1 PCR products of non-targeted Saccostrea species DNA (same PCR as 3a but run in a different gel); and c Saccostrea multiplex 2 PCR products of targeted Saccostrea species DNA amplified at an annealing temperature of 62 °C. NC = negative control. See Supplementary Figures S4 – S6 for original photographs