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Table 1 PERMANOVA results for dinoflagellate assemblage alpha diversity (observed richness, Shannon Diversity, and Dominance and Rarity Indices) and assemblage structure throughout the water column to a depth of 200 m beneath the sea surface at depth zones (D1: 5–10 m, D2: 20–30 m, D3: 45–60 m, D4: 95–120 m, D5: 130–150 m) at Station A, B, and C. Alpha diversity data were raw counts. Assemblage composition data were normalized to median sequencing depth and Hellinger-transformed before analysis. Significant effects at p < 0.05 are highlighted in bold

From: eDNA metabarcoding reveals biodiversity and depth stratification patterns of dinoflagellate assemblages within the epipelagic zone of the western Coral Sea

Diversity metric

Factor

Df

Sum of squares

R2

F

p

Assemblage structure

Depth

Station

Residual

Total

4

2

8

14

1.548

0.693

1.790

4.030

0.384

0.172

0.443

1

1.73

1.55

0.003

0.037

Dominance index

Depth

Station

Residual

Total

4

2

8

14

0.134

0.004

0.026

0.163

0.819

0.023

0.156

1

10.46

0.597

0.004

0.576

Rarity index

Depth

Station

Residual

Total

4

2

8

14

0.160

0.020

0.054

0.234

0.683

0.086

0.229

1

5.96

1.51

0.020

0.313

Observed richness

Depth

Station

Residual

Total

4

2

8

14

53,341

154,683

502,666

710,689

0.075

0.217

0.707

1

0.21

1.23

0.927

0.376

Shannon diversity

Depth

Station

Residual

Total

4

2

8

14

1.069

0.701

2.058

3.829

0.279

0.183

0.537

1

1.04

1.36

0.397

0.302