Fig. 3
From: Emerging roles for diguanylate cyclase during the evolution of soma in dictyostelia

Expression pattern ofPvio dgcA. The 2.8 kb 5’-intergenic region of Pvio dgcA was fused to lacZ and expressed in Pvio wild-type. The cells were plated for development until the indicated stages were reached, and then fixed and stained with X-gal. a, early aggregate; b, late aggregate; c, primary sorogen; d, tip formation in segregating whorls; e, secondary sorogens. Scale bars: 0.1 mm. Representative images from two different experiments of two separately developed clones are shown