Table 1 The potential functional effect of main amino acid substitutions at HPSs (x—indicate positive selection by the selection test)
Site | Ancestral amino acid | Alternative amino acid | Domain | PAML | DATAMONKEY | Provean | DYNAMUT | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
|  | SLAC | FEL | MEME | FUBAR | Score | Prediction (cutoff = -2.5) | ΔΔG(kcal/mol) | Action | ||||
35 | Ile | Leu | Trans | - | - | - | x | - | -0.913 | Neutral | 0.372 | Stabilizing |
Ile | Val | Trans | -0.370 | Neutral | -0.371 | Destabilizing | ||||||
97 | Thr | Ile | Trans | - | - | - | - | - | 2.683 | Neutral | 1.045 | Stabilizing |
147 | Val | Ile | Trans | - | - | - | - | - | 0.396 | Neutral | 0.677 | Stabilizing |
153 | Ala | Ser | Trans | - | - | - | - | - | -0.776 | Neutral | -1.088 | Destabilizing |