Fig. 6

Detection of positive selection on several amino acid sites in F3'H2 and DFR3 in Ruellia and predicted effects on protein function. Upper Panels (both genes). Amino acid alignment positions shown at top. Black, dashed line with red dots denote sites under positive selection based on analyses implementing a M1 + M2 and M7 + M8 model of evolution. Protein binding regions (red diamonds) containing sites under positive selection marked with blue frames. yellow/orange circles stand for predicted nucleotide binding region, and the pink and blue rectangles stand for helex and strand respectively. Lower Panels (both genes). Expanded, view of blue frames showing amino acid substitutions and sites under positive selection, these marked by red triangles at bottom of alignment. To the right, predication of functional effects of sequence variants conducted using R. simplex as a reference. Sites under positive selection marked by red triangles and highlighted with a black outline. Dark red (scores > 50): strong signal for a potential functional effect; white (scores < 50 or > −50): weak signal for potential effect; green (scores < −50): neutral or no effect