Table 2 Oligonucleotide primers for PCR amplification of whitefly mitochondrial DNA fragments.a
Primer | Gene | Position on AY5212656 | Nucleotide sequence of conserved regions (5'->3') |
|---|---|---|---|
F-COI-1 | COI | 172–221 | TCWCATGCWT TTATYATAAT TTTTTTYATR ACWATGCCTT TDGTWATTGG |
F-COI-2 | COI | 673–710 | GAYCCHATTT TRTATCAACA YTTDTTTTGA TTTTTTGG |
R-COI | COI | 1133–1069 | ACATAATGAA AATGDGCAAC AACAAAATAW GTATCATG HA RACAHACATC HACHGAAGAA TTACC |
F-COII | COII | 1845–1876 | CCTTCTATYC GDATTTTDTA TYTAATRGAT GA |
R-COII | COII | 2093–2067 | AGGAACHGTY CAAGAATGHA AAACATC |
F-COIII | COIII | 3854–3879 | TTAACWGGHT TTCAYGGNTT HCATGT |
R-COIII | COIII | 4002–3974 | CARACWAHRT CDACRAAATG TCAGTATCA |
F-CYTB | CYTB | 9169–9215 | GCTTTTATRG GBTATATYTT RCCTTGRGGY CARATATCTT TTTGRGG |
R-CYTB | CYTB | 9566–9544 | GCTATAATAA AATTTTCTGA ATC |
F-ND1 | ND1 | 10275–10314 | ATTCAATRTT AAAWCCWGAA ATWARYTCTG AYTCTCCTTC |
R-ND1 | ND1 | 10506–10484 | CAAYTAATTT CDTATGAAAT TAA |
F-16S-1 | 16S | 11053–11087 | ACCTGGCTTA CGCCGGTCTG AACTCAGATC ATGTA |
F-16S-2 | 16S | 11202–11220 | GCTGTTATCC CTTAGGTAA |
R-16S-1 | 16S | 11377–11354 | AAAAGACAAR AAGACCCTTT AGAA |
R-16S-2 | 16S | 11526–11483 | TTAAATAGCT GCAGTAWATT DACTGTACTA AGGTAGC ATA ATAA |
F-12S-1 | 12S | 12273–12308 | ACTTTCCAGT AADTTTACTT TGTTACGACT TATCTT |
F-12S-2 | 12S | 12318–12342 | AAGAGTGACG GGCRATTTGT ACATA |
R-12S-1 | 12S | 12643–12619 | CTTCAAACTT AAAAAATTTG GCGGT |
R-12S-2 | 12S | 12861–12843 | GTGCCAGCAG TWGCGGTTA |