Structural, functional, and evolutionary analysis of moeZ, a gene encoding an enzyme required for the synthesis of the Pseudomonas metabolite, pyridine-2,6-bis(thiocarboxylic acid)

Table 2 Characteristics, similarities, and homologies of sequences used in this study.

Designation	Length		ThiF Domain BLAST¹	RHOD Domain BLAST¹	AA similarity to Ps-MoeZ			NA homology to Ps-moeZ
	NA	AA			BLAST²	Identity	AA Overlap	Identity^2,3	Overlap (bp)⁴
Ec-moeB	747	249	e^-35	NA	e^-42	39%	245	0	NA⁵
Mlo-moeZdR	813	271	e^-39	NA	e^-66	52%	248	91%	35
Mle-moeZ	1185	395	e^-40	e^-10	e^-121	57%	391	75%	474
Mt-moeBR	1167	389	e^-43	e^-13	e^-103	50%	385	0	NA⁵
Mt-moeZ	1176	392	e^-39	e^-8	e^-121	57%	391	78%	486
Nos-moeZ	1170	390	e^-42	e^-9	e^-95	49%	379	0	NA⁵
Pa-moeB	762	254	e^-30	NA⁵	e^-53	48%	245	80%	65
Pa-moeBR	1176	392	e^-42	e^-7	e^-74	41%	389	79%	202
Ps-moeZ	1173	391	e^-39	e^-9	NA⁵	NA⁵	NA⁵	NA⁵	NA⁵
Sc-moeBR	1176	392	e^-34	e^-10	e^-114	54%	396	81%	325
Syc-moeZ	1173	391	e^-42	e^-9	e^-101	53%	391	79%	286
Syy-moeZ	1176	392	e^-31	e^-8	e^-93	47%	390	0	NA⁵

¹ThiF and RHOD domain E value exponential components reported as part of the CD results of standard protein BLAST (blastp) search. ²AA similarity and NA homology E value exponential components as reported by BLAST 2 sequences. ³When genes had more than one segment of nucleic acid homology, the % identity values were determined by averaging the % identity of all segments. ⁴When genes had more than one segment of nucleic acid homology, the lengths of these segments were added together. ⁵NA = Not applicable.

ISSN: 2730-7182